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credependent calcium indicator pgp aav1 syn flex jgcamp7s wpre  (Addgene inc)


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    Addgene inc credependent calcium indicator pgp aav1 syn flex jgcamp7s wpre
    Credependent Calcium Indicator Pgp Aav1 Syn Flex Jgcamp7s Wpre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgp+aav+syn+flex+jgcamp7s/pGP-AAV-syn-FLEX-jGCaMP7s-WPRE+(Plasmid+%23104491)/pm41887592-77-16-22
    Average 95 stars, based on 77 article reviews
    credependent calcium indicator pgp aav1 syn flex jgcamp7s wpre - by Bioz Stars, 2026-10
    95/100 stars

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    Plasmid Preparation:

    Article Title: Gephyrin filaments represent the molecular basis of inhibitory postsynaptic densities
    Article Snippet: .. mScarlet-Gphn C4c from pAAV-hSyn-fDIO-mScarlet-Gphn_C4c (Addgene plasmid # 194974 ; RRID:Addgene_194974) and pAAV-hSyn-fDIO-mScarlet-Gphn_P1 (Addgene plasmid # [ 194972 ]; RRID:Addgene_194972) were initially subcloned into pGP-AAV-syn-FLEX-jGCaMP7s, in which the WPRE was removed using HindIII and NotI (pGP-AAV-syn-FLEX-jGCaMP7s-WPRE was a gift from Douglas Kim & GENIE Project (Addgene plasmid # 104491 ; RRID:Addgene_104491)) using NEBuilder HiFi DNA Assembly (NEB). .. Since one of the AAV2 ITR sites was destroyed in the process, the inserts mScarlet-Gphn C4c and mScarlet-Gphn_P1, including loxP and lox2272 sites, were subcloned into the backbone of pAAV-hSyn-fDIO-mScarlet-Gphn_P1 (Addgene plasmid # 194972 ; RRID:Addgene_194972) using HindIII and Eco47III.



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    A mouse model of a two-option probabilistic reward choice task (PRT). Male GAD65-Cre mice (n = 5 mice) and their wild-type littermates (n = 2 mice) were trained in an operant task designed to assess value-based decision-making. (A) Schematic of behavioral chambers used in the PRT. In Option A, a nose-poke made into aperture A resulted in a 75% chance of receiving a high-value reward (Ensure, ∼24 μl, delivered over 2 s) and a 25% chance of receiving the same amount of water. Both liquids were delivered via a composite spout located at the lower-right corner. In Option B, a nose-poke into aperture B led to a 25% chance of receiving Ensure and a 75% chance of receiving water. The timing of each licking on the spout was recorded with a lickometer. (B) Actual recorded probabilities of receiving Ensure following Choice A (set at 75%) and Choice B (set at 25%), confirming task implementation. (C) Choice behavior across 5 training sessions (Days 1-5). Mice increasingly favored Choice A. Two-way ANOVA with repeated measures shows a significant main effect of choice (F(1,12) = 33.7, P = 8.4 × 10 -5 ), and a significant effect of training session (F(4,24) = 5.46, P = 0.0029). N = 7 mice. (D) Total number of nose-pokes across the five sessions. A trend toward increased nose-poking was observed, but the session effect did not reach statistical significance (F(4,24) = 2.41, P = 0.078).

    Journal: bioRxiv

    Article Title: A ventral tegmental area GABAergic projection to the ventral pallidum regulates value-based decision making in mice

    doi: 10.64898/2026.02.19.706858

    Figure Lengend Snippet: A mouse model of a two-option probabilistic reward choice task (PRT). Male GAD65-Cre mice (n = 5 mice) and their wild-type littermates (n = 2 mice) were trained in an operant task designed to assess value-based decision-making. (A) Schematic of behavioral chambers used in the PRT. In Option A, a nose-poke made into aperture A resulted in a 75% chance of receiving a high-value reward (Ensure, ∼24 μl, delivered over 2 s) and a 25% chance of receiving the same amount of water. Both liquids were delivered via a composite spout located at the lower-right corner. In Option B, a nose-poke into aperture B led to a 25% chance of receiving Ensure and a 75% chance of receiving water. The timing of each licking on the spout was recorded with a lickometer. (B) Actual recorded probabilities of receiving Ensure following Choice A (set at 75%) and Choice B (set at 25%), confirming task implementation. (C) Choice behavior across 5 training sessions (Days 1-5). Mice increasingly favored Choice A. Two-way ANOVA with repeated measures shows a significant main effect of choice (F(1,12) = 33.7, P = 8.4 × 10 -5 ), and a significant effect of training session (F(4,24) = 5.46, P = 0.0029). N = 7 mice. (D) Total number of nose-pokes across the five sessions. A trend toward increased nose-poking was observed, but the session effect did not reach statistical significance (F(4,24) = 2.41, P = 0.078).

    Article Snippet: For fiber photometry studies, GAD65-Cre mice were infused with pGP-AAV1-syn-FLEX-jGCaMP7s-WPRE (Addgene, plasmid #104491) into the right VTA.

    Techniques: